Objective: To study agents of animal wound myiasis in various geographical districts of Fars province.Methods:of 10358 domestic animals have been visited from April 2011 to March 2012. The infected wounds in any parts of animal body were sampled by means of forceps.Results:This study has been done in Fars province, located in the south part of Iran. Sums The most wound myiasis cases due to this species occurred in central part of Fars province. There wasn’t any significant difference between sheep and goat in infestation with myiasis (P>0.05). The infestation rate of myiasis in cattle community was 0.86%. About 61% of all animal wound myiasis were caused by larvae of Wohlfahrtia magnifica. Conclusions: The infestation rate of livestock was lower than other works in Iran and some other countries like Saudi Arabia. Chrysomya bezziana has been mentioned as main myiasis agent in Iran. But in this study it cleared that similarly to some European countries, the common animal myiasis agent in Iran is Wohlfahrtia magnifica. Introducing new species as principal agent for myiasis can help public health and animal husbandry policy makers to prepare sufficient and effective control and/or preventive measures for this disease.