采用国外新向世的反相聚合柱分离测定了人血清结合型和游离型胆汁酸。研究了胆汁酸在PRP-1柱的保留特性;使用pH10。0的磷酸缓冲液、乙腈,甲醇组成的三元淋洗剂,确保15种胆汁酸的分离。还进行了方法回收率、重复性、最低检知量、线性范围试验,方法可靠、精确。分析了60例血清胆汁酸,获得各种肝胆疾病患者血清胆汁酸特征图谱。 Human serum-associated and free-form bile acids were isolated using a new reversed-phase polymerization column from abroad. The retention characteristics of bile acids in PRP-1 column were studied. The trivalent elution agent consisting of pH 10.0 phosphate buffer, acetonitrile and methanol was used to ensure the separation of 15 bile acids. The method recovery rate, repeatability, minimum detection amount, and linear range test were also performed. The method was reliable and accurate. 60 cases of serum bile acids were analyzed to obtain the characteristics of serum bile acids in patients with various hepatobiliary diseases.