35例孕妇(妊娠14～24周)的羊水标本,采用199混合培养液培养,羊水细胞生长良好。制片观察获得较好的效果。羊水内胎儿细胞生长的好坏取决于羊水采集时间、混合培养基的成份、pH值和培养过程等多种因素,其中培养基pH值和羊水pH值应接近是关键因素之一。羊水内有少量红细胞对羊水细胞生长无明显影响。 35 cases of pregnant women (14 to 24 weeks of gestation) of amniotic fluid specimens, using 199 mixed culture medium, amniotic fluid cells grow well. Observe the production of better results. Amniotic fluid fetal cell growth depends on the amniotic fluid collection time, mixed medium composition, pH and culture process and other factors, including medium pH and amniotic fluid pH value should be close to one of the key factors. Amniotic fluid within a small amount of red blood cells on the amniotic fluid cell growth no significant effect.