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害虫对杀虫剂的抗性遗传特性是农林业害虫防治体系中制定抗性治理对策的重要依据。以鳞翅目昆虫家蚕作为模型,研究害虫对微生物杀虫剂苏云金芽孢杆菌(Bt)毒素的抗性遗传特性。在调查100个家蚕品系对Bt毒素Cry1Ac的抵抗能力的基础上,运用经典的杂交和回交方法,以家蚕抗性品系ACR1(R,LC50>375 mg/L)和敏感品系Nistari(S,LC50=37.5 mg/L)为亲本建立正反交F1、自交F2及回交BC1群体,进一步分析家蚕对Bt毒素Cry1Ac的抗性遗传模式。通过对供试家蚕群体2龄幼虫添食Bt毒素Cry1Ac后24 h的死亡与存活表型分离数据进行统计分析,最终确定家蚕抗性品系ACR1对Bt毒素Cry1Ac的抗性由隐性单基因控制。
Pest insecticide resistance genetic characteristics of the agricultural and forestry pest control system to develop an important basis for resistance control measures. The lepidopteran insects silkworm as a model to study the pest insects on the microbial insecticide Bacillus thuringiensis (Bt) toxin genetic characteristics. Based on the investigation of resistance to Bt toxin Cry1Ac in 100 silkworm strains, the silkworm resistance strain ACR1 (R, LC50> 375 mg / L) and the susceptible strain Nistari (S, LC50 = 37.5 mg / L) were used as parents to establish F1, F2 and backcross BC1 populations. The inheritance patterns of Bt toxin Cry1Ac in silkworm were further analyzed. Through statistical analysis of the phenotypic data of death and survival at 24 h after Bt toxin Cry1Ac was inoculated into the second instar larvae of the tested silkworm population, the resistance of the silkworm resistant strain ACR1 to Bt toxin Cry1Ac was finally determined by the recessive single-gene control.