We aimed to establish a novel rat model of seminal vesiculitis that would provide an effective approach to investigate the pathogenesis of this disease in the future.Eight male rats received the same operation,during which the root of one of the two seminal vesicles was partly ligatured with sutures and the other vesicle was left intact.The samples of seminal vesicles were harvested on the 8th day following the operation.Hematoxylin and eosin and Masson\'s trichrome stains were used to observe the histopathology and the presence of fibrous tissue in seminal vesicles,respectively.Immunoblotting and immunohistochemistry were applied to determine the tumor necrosis factor-alpha and cyclooxygenase-2 levels in seminal vesicle tissues.Real-time fluorescence quantitative polymerase chain reaction was performed to measure the gene expression levels of proinflammatory cytokines.H2O2 levels in the seminal plasma from the seminal vesicle were also measured.Hematoxylin and eosin staining suggested that there was inflammatory cell infiltration into the seminal vesicles treated by partial root ligation.The tumor necrosis factor-alpha and cyclooxygenase-2 proteins were significantly upregulated in the treated seminal vesicles.The tumor necrosis factor-alpha,cyclooxygenase,interleukin 6,and inducible nitric oxide synthase mRNA expression levels were also upregulated in the treated seminal vesicles.The H2O2 levels in the seminal plasma from seminal vesicles with partial root ligation were significantly elevated compared with those from vesicle left intact.In conclusion,partially ligating the root of the seminal vesicle via sutures in rats is an effective method to establish a seminal vesiculitis rat model.