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目的:观察当归贝母苦参煎剂对慢性细菌性前列腺炎(CBP)大鼠前列腺组织中肿瘤坏死因子-α(TNF-α)的免疫组化和mRNA表达的影响,探讨其可能的作用机制。方法:雄性SD大鼠60只,随机分为空白对照组、模型组、诺氟沙星组、当归贝母苦参煎剂高、中、低剂量组6组,每组10只,前列腺背叶注射大肠埃希菌制备CBP动物模型。当归贝母苦参煎剂高、中、低剂量分别为11.67,5.83,2.92 g.kg-1,诺氟沙星剂量为9.3×10-2g.kg-1。术后第15天起用药,连续4周。用免疫组化法观测大鼠前列腺组织中的TNF-α表达,用RT-PCR法测大鼠前列腺组织TNF-αmRNA的表达。结果:与模型组比较,当归贝母苦参煎剂各剂量组TNF-α免疫组化阳性表达和mRNA基因表达均明显降低(P<0.01),其中高剂量组最低。结论:当归贝母苦参煎剂治疗慢性细菌性前列腺炎的作用机制可能与减少TNF-α的产生有关。
OBJECTIVE: To observe the effect of FGP on the immunohistochemistry and mRNA expression of tumor necrosis factor-α (TNF-α) in prostate tissue of chronic bacterial prostatitis (CBP) rats and to explore its possible mechanism . Methods: Sixty male Sprague-Dawley rats were randomly divided into 6 groups (blank control group, model group, norfloxacin group, Injecting Escherichia coli to prepare CBP animal model. Angelica Fritillaria decoction of high, medium and low doses were 11.67,5.83,2.92 g.kg-1, norfloxacin dose of 9.3 × 10-2g.kg-1. After the first 15 days from the medication for 4 weeks. The expression of TNF-αin rat prostate tissue was detected by immunohistochemistry, and the expression of TNF-αmRNA in rat prostate was detected by RT-PCR. Results: Compared with the model group, the positive expression of TNF-α and the mRNA expression of TNF-α in each dose group were significantly decreased (P <0.01), and the highest dose group was the lowest. Conclusion: Angelica Fritillaria decoction for the treatment of chronic bacterial prostatitis mechanism may be related to reducing the production of TNF-α.