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研究6种饵料微藻:微绿球藻(Nannochloropsis oculata)、小球藻(Chlorella.sp)、球等鞭金藻(Isochrysis galbana)、三角褐指藻(Phaeodactylum tricornutum)、亚心形扁藻(Platymonas subordi-formis)、中肋骨条藻(Skeletonema costatum)对双刺纺锤水蚤(Acartiabifilosa)雌体繁殖的影响,以探索适合培养双刺纺锤水蚤的藻类。应用解剖镜下计数方法对雌体存活率、产卵率、孵化率、排粪率几个指标进行检测。结果表明微绿球藻、小球藻和球等鞭金藻明显不适于用作雌体饵料;中肋骨条藻组产卵率最高,亚心形扁藻和三角褐指藻组的产卵率没有显著差异;亚心形扁藻组的排粪率明显低于中肋骨条藻和三角褐指藻组;摄食三角褐指藻的雌体所产的卵其平均孵化率要显著低于亚心形扁藻和中肋骨条藻;亚心形扁藻组雌体的产卵率尽管较中肋骨条藻组低,但却保持最高的雌体存活率,而且排粪率也要比中肋骨条藻低得多。综合比较得出结论:6种饵料微藻中,亚心形扁藻和中肋骨条藻是两种最适宜培养双刺纺锤水蚤雌体的优良饵料,二者混合使用效果会更好。
Six species of food microalgae were studied: Nannochloropsis oculata, Chlorella sp., Isochrysis galbana, Phaeodactylum tricornutum, Platymonas subordi-formis, and Skeletonema costatum on the reproduction of Acartiabifilosa females in order to explore the suitable algae for the culture of Spinibarbus Daphnia. The application of anatomic microscope counting method to test the survival rate, oviposition rate, hatching rate and defecation rate of female body. The results showed that Microgreen algae, Chlorella vulgaris and Isochrysis galbanum were obviously not suitable for female body bait. The oviposition rate of Skeletonema costatum was the highest, No significant difference was found; the flatulence rate of P. alga was significantly lower than that of Skeletonema costatum and T. trifoliatus; the average hatching rate of the eggs produced by female T. cruciflorus was significantly lower than that of the sub-heart Platymonas subcordyloe and Skeletonema costatum. Although the spawning rate of female P. avatar was lower than that of S. striatella, the highest female body survival rate was maintained and the defecation rate was lower than that of middle rib cage Algae is much lower. The results showed that among the six kinds of food microalgae, Platymonas subcordulans and Skeletonema costatum were the best two kinds of feed for cultivating female Spodoptera diterpenensis, and the combination of them would be better.