论文部分内容阅读
番茄黄化曲叶病毒病是番茄生产中的一种毁灭性病毒病害,2009年传入北京。利用烟粉虱传双生病毒简并引物PA/PB对2010年～2011年采集自北京市5个区县的53个番茄样品进行检测,30个表现典型黄化曲叶病症状的样品均扩增得到约500 bp的特异条带,测定了其中7个样品的部分序列,经序列比对分析表明其为番茄黄化曲叶病毒(Tomato yellow leafcurl virus,TYLCV)。利用TYLCV特异引物TJ-F/TJ-R、TY-F/TY-R对样品BJDXXY、BJFS02、BJFS03、BJMY2231进行TYLCV基因组克隆和序列测定,经分析4个样品携带的TYLCV基因组长度均为2 781碱基,编码6个蛋白。基因组序列比较发现,这4个分离物与TYLCV-Israel株系同源性达到98%以上;通过建立系统发育树,发现BJDXXY、BJFS02、BJFS03与河北分离物(HBLF4)、山东分离物(SDSG)亲缘关系较近,BJMY2231与上海分离物(TYLCV-Israel)、江苏分离物(JSNJ1)亲缘关系较近。
Tomato yellow leaf curl virus disease is a devastating virus disease in tomato production, introduced to Beijing in 2009. Fifty-three tomato samples collected from five districts and counties in Beijing from 2010 to 2011 were detected using PA / PB, a dengue virus-derived dengue virus, and 30 samples of typical symptoms of yellow leaf curl were amplified Specific bands of about 500 bp were obtained. Seven of these samples were sequenced. Sequence alignment analysis showed that they were Tomato yellow leaf curl virus (TYLCV). TYLCV genomic cloning and sequencing of samples BJDXXY, BJFS02, BJFS03 and BJMY2231 were carried out by using TYLCV specific primers TJ-F / TJ-R and TY-F / TY-R. The length of TYLCV genome in 4 samples was 2 781 Base, encoding six proteins. Genomic sequence comparison showed that these four isolates shared more than 98% homology with TYLCV-Israel strains. Through the establishment of phylogenetic tree, BJDXXY, BJFS02 and BJFS03 were found to be highly homologous with Hebei isolate (HBLF4), Shandong isolate (SDSG) The close relationship between BJMY2231 and TYLCV-Israel, Jiangsu isolates (JSNJ1) was closer.