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用肺灌洗法获取狗肺泡巨噬细胞(AM),并进行体外培养,观察铀矿粉、石英粉对AM膜的过氧化作用及五味子丙素、V_E对生物膜的保护效果。此外,还进行了用一次性粉尘气管内注入使大鼠染尘形成实验性矽肺的体内研究,以观察V_E对整体实验动物抗氧化作用。结果表明:两种粉尘均能诱发膜脂质过氧化,五味子丙素和V_E有明显的抗氧化作用;V_E体内给药90天,分期分批杀死动物,分别称出肺干、湿重,测定肺脂质过氧化产物丙二醛和肺胶原蛋白,用光镜、电镜观察组织病理学改变,并比较了阳性对照组与联合用药组的测定结果,证明V_E在染尘早期有抑制两种粉尘诱导的膜非饱和酸(Fu-FA)的过氧化,阻断自由基的链锁反应,以此能延缓矽肺病变发展,但V_E单一用药逊于V_E与磷酸羟基哌喹联合用药的效果。
Lung alveolar macrophages (AM) were obtained by pulmonary lavage and cultured in vitro. The peroxidation of uranium ore powder and quartz powder on AM film and the protective effect of Schisandrin CP and V_E on biofilm were observed. In addition, an in vivo study of dust formation in experimental silicosis in rats by intraductal administration of disposable dust was also performed in order to observe the antioxidant effect of V_E on the whole experimental animals. The results showed that both of the two kinds of dust could induce membrane lipid peroxidation, and Schisandrin C and V_E had obvious anti-oxidant effect. V_E was administrated for 90 days in vivo and the animals were killed in batches in batches, The lung lipid peroxidation products malondialdehyde and lung collagen were measured. The histopathological changes were observed by light microscope and electron microscope. The results of the positive control group and the combination group were compared. Dust-induced peroxidation of membrane-bound unsaturated acids (Fu-FA) blocked the free radical chain reaction, which delayed the development of silicotic lesions, but the single-use of V_E was inferior to the combination of V_E and hydroxyperperoxid.