在玻璃微流控芯片通道表面用硅烷化试剂二氯二甲基硅烷进行处理后,二氯二甲基硅烷与玻璃通道表面的硅羟基反应,硅烷基覆盖在原来的硅羟基上,其结果为电渗流减小甚至完全消除.进一步采用全势能线性糕模轨道分子动力学方法,对表面反应的微观结构进行了理论计算,计算结果表明硅羟基中的氢原子与二氯二甲基硅烷中的氯原子结合形成稳定的HCl分子结构而脱离,从而使硅烷基覆盖在表面上. Dichlorodimethylsilane reacts with the silanol groups on the surface of the glass channel after the silanization reagent dichlorodimethylsilane is treated on the channel surface of the glass microfluidic chip and the silyl groups are covered on the original silanol groups. The electroosmotic flow is reduced or even eliminated completely.The microstructure of the surface reaction is further calculated by the method of full potential linear cakes orbital molecular dynamics.The calculation results show that the hydrogen atoms in the silanol groups and the dichlorodimethylsilane Chlorine atoms combine to form a stable molecular structure of HCl, leaving the silane groups on the surface.