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以2份抗病及2份感病玉米自交系为试验材料,模拟玉米丝黑穗病适宜的侵染及田间发病条件,采用室内菌土接种方法培养玉米幼苗,通过PCR检测病原菌侵染率,研究苗期PCR检测抗、感玉米自交系的最佳取样时期和检测部位。结果表明,SSR标记SR3可用于玉米丝黑穗病室内接种鉴定;同一自交系不同的取样时期叶鞘侵染率均显著高于叶片侵染率;感病自交系与抗病自交系三叶期第3叶叶鞘的PCR侵染率差值极显著高于其他取样时期和取样部位,且该期4份自交系的侵染率与田间接种条件下的发病率呈显著正相关,为鉴别抗感自交系最佳的取样时期和部位。
Two lines of disease-resistant and two susceptible maize inbred lines were used as experimental materials to simulate maize smut infection and field conditions. Maize inoculation method was used to inoculate maize seedlings. The infection rate of pathogen , To study the seedling PCR detection of anti-susceptible maize inbred lines of the best sampling period and test site. The results showed that the SSR marker SR3 could be used for the identification of maize head smut. The rates of leaf sheath infection in different inbred lines were significantly higher than those in the same inbred lines. The susceptible inbred lines and resistant inbred lines The difference in PCR infection rate of the third leaf sheath at the leaf stage was significantly higher than that at other sampling and sampling sites. The infection rates of the 4 inbred lines at this stage were significantly and positively correlated with the incidence of field inoculation, and were Identification of anti-susceptible inbred lines of the best sampling period and location.