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目的:分析核转录因子κB p65(NF-κBp65)和核转录因子κB抑制蛋白α(IκBα)在正常早孕绒毛及妊娠滋养细胞疾病中表达的差异性,以及与妊娠滋养细胞肿瘤(GTN)(包括侵蚀性葡萄胎和绒毛膜癌)患者年龄及临床分期的关系。并探讨两者在妊娠滋养细胞肿瘤组织中的相关性。方法:采用免疫组化方法(SP法)检测20例正常早孕绒毛组织、30例葡萄胎组织、13例侵蚀性葡萄胎和15例绒毛膜癌中NF-κBp65和IκBα的表达情况。结果:NF-κBp65在正常早孕绒毛组与侵蚀性葡萄胎组、正常早孕绒毛组与绒毛膜癌组、葡萄胎组与侵蚀性葡萄胎组、葡萄胎组与绒毛膜癌组之间阳性表达率比较差异均具有统计学意义(P=0.013,0.018,P=0.026,0.035,P<0.05);IκBα在正常早孕绒毛组与侵蚀性葡萄胎组、正常早孕绒毛组与绒毛膜癌组、葡萄胎组与侵蚀性葡萄胎组、葡萄胎组与绒毛膜癌组之间阳性表达率比较差异均具有统计学意义(P<0.01)。NF-κBp65和IκBα在GTN中的表达与临床分期有关(P=0.043,0.042,P<0.05),与患者年龄无关。NF-κBp65与IκBα在GTN中呈负相关(r=-0.403,P=0.034,P<0.05)。结论:NF-κBp65上调、IκBα的下降或缺失可能与滋养细胞肿瘤的发生发展以及侵袭、转移有关,NF-κBp65、IκBα两者在妊娠滋养细胞肿瘤组织中的表达呈负相关。
OBJECTIVE: To analyze the differences in expression of NF-κB p65 (NF-κBp65) and NF-κB alpha in normal early gestational trophoblastic disease and gestational trophoblastic disease (GTN), including Invasive hydatidiform mole and choriocarcinoma) in patients with age and clinical stage of the relationship. And to explore the two in gestational trophoblastic tumor tissue correlation. Methods: The expression of NF-κBp65 and IκBα in 20 cases of normal early pregnancy villi, 30 cases of hydatidiform mole, 13 cases of invasive mole and 15 cases of choriocarcinoma were detected by immunohistochemistry (SP method). Results: The positive expression rate of NF-κBp65 between normal early pregnancy villus group and invasive hydatidiform mole group, normal early villus group and choriocarcinoma group, hydatidiform mole group and invasive hydatidiform mole group, hydatidiform mole group and choriocarcinoma group (P = 0.013,0.018, P = 0.026, 0.035, P <0.05). The expression of IκBα in normal early villi and invasive hydatidiform mole group, normal early villus and choriocarcinoma group, hydatidiform mole There were significant differences in the positive expression rates between the hydatidiform mole group and the invasive hydatidiform mole group, the hydatidiform mole group and the choriocarcinoma group (P <0.01). The expression of NF-κBp65 and IκBα in GTN was correlated with clinical stage (P = 0.043,0.042, P <0.05), but not with patient’s age. NF-κBp65 was negatively correlated with IκBα in GTN (r = -0.403, P = 0.034, P <0.05). CONCLUSION: The up-regulation of NF-κBp65 and the down-regulation or loss of IκBα may be related to the occurrence and development of trophoblastic tumor, invasion and metastasis. The expression of NF-κBp65 and IκBα in gestational trophoblastic tumor tissue is negatively correlated.