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目的观察致死剂量微波辐照后小鼠肝超微结构及热休克蛋白70(HSP70)表达的变化,探讨其在法医学鉴定中的意义。方法将昆明种小鼠分为正常对照组和辐照组,辐照组小鼠采用峰值功率密度为129 W/cm~2的微波全身一次辐照30 min致小鼠死亡后,立即用透射电镜观察小鼠肝的超微结构变化;采用反转录-聚合酶链反应(RT-PCR)法检测小鼠肝HSP70 mRNA表达变化,免疫印迹法检测小鼠肝HSP70蛋白表达变化。结果致死剂量微波辐照后小鼠肝细胞胞浆点片状溶解,线粒体嵴和膜溶解消失;HSP70 mRNA和蛋白水平表达显著上调,与正常组对照比较,差异具有极显著统计学意义(P<0.01)。结论微波辐照致死可导致小鼠肝细胞胞浆溶解、线粒体损伤、HSP70 mRNA和蛋白水平表达明显上调,可作为微波辐照致死鉴定的重要参考指标。
Objective To observe the changes of hepatic ultrastructure and the expression of heat shock protein 70 (HSP70) after lethal dose microwave in mice, and to explore its significance in forensic identification. Methods Kunming mice were divided into normal control group and irradiation group. The mice in irradiation group were exposed to a whole body radiation with a peak power density of 129 W / cm ~ 2 for 30 min. After the mice died, they were examined by transmission electron microscopy The ultrastructural changes in the liver of the mice were observed. The expression of hepatic HSP70 mRNA in the liver of the mice was detected by reverse transcription-polymerase chain reaction (RT-PCR), and the expression of hepatic HSP70 was detected by Western blotting. Results After exposure to lethal dose of microwave, the cytoplasmic lysis of hepatocytes was abolished and the mitochondrial cristae and membrane were dissolved. The expression of HSP70 mRNA and protein were significantly up-regulated. Compared with the control group, the difference was statistically significant (P < 0.01). Conclusion The result of microwave irradiation could cause cytolysis, mitochondrial damage and expression of HSP70 mRNA and protein in hepatocytes of mice, which may serve as an important reference index for the identification of lethality induced by microwave irradiation.