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目的了解宁波地区致泻性病原菌组成和主要流行株的基因分型,探讨菌株的同源性,为追踪传染源提供依据。方法病原菌检测采用直接与增菌分离相结合的方法;细菌鉴定采用生化筛检和API等法;细菌分型采用诊断血清和PFGE分型。结果本组9 256份样本检出8类16种3 473株致泻性病原菌,检出率为37.52%,以副溶血性弧菌最高,与其他病原菌比差异有统计学意义(P<0.05);血清分型发现副溶血性弧菌的O3群、沙门菌的甲型副伤寒、志贺菌的褔氏志贺菌、致病性大肠埃希菌O119为各病原菌的优势菌;PFGE分型副溶血性弧菌分出19个,伤寒沙门菌分出3个,甲型副伤寒沙门菌分出12个。结论副溶血性弧菌是引起宁波地区感染性腹泻最主要的流行株,PFGE分型能显示菌株间的亲缘关系,PFGE分子分型可分析菌株的来源,为流行病学追踪传染源提供依据。
Objective To understand the composition of diarrheal pathogens and the genotyping of major epidemic strains in Ningbo, and to explore the homology of the strains for the purpose of tracking the source of infection. Methods The pathogen detection method was directly combined with the method of enrichment and separation. The method of biochemical screening and API was used for bacterial identification. The bacterial typing was performed by using the diagnostic serum and PFGE typing. Results Among 9 256 samples of 8 groups, 3 473 strains of pathogenic bacteria with 3 473 diarrhea were detected. The detection rate was 37.52%. The highest ratio of Vibrio parahaemolyticus to other pathogens was found (P 0.05) ; Serological typing found Vibrio parahaemolyticus O3 group, Salmonella paratyphi A, Shigella Shigella Shigella, pathogenic Escherichia coli O119 for the dominant bacteria; PFGE type 19 strains of Vibrio parahaemolyticus, 3 of Salmonella typhi, and 12 of Salmonella paratyphi A were isolated. Conclusion Vibrio parahaemolyticus is the most prevalent epidemic strain of infectious diarrhea in Ningbo. PFGE typing can show the genetic relationship among isolates. PFGE molecular typing can analyze the origin of the isolates and provide a basis for epidemiology to follow the source of infection.