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本试验通过从枯死松树分离繁殖的松材线虫 Bursaphelenchus xylophilus 接种3～4年生马尾松苗,观察到接种株第45天开始出现外观症状,三个月内接种株相继枯死。在枯死株中分离出大量松材线虫,表明松材线虫对马尾松具有明显的致病性。试验还通过酸性品红染色追踪树液流,观察到松材线虫侵染早期,其松脂管的薄壁细胞受破坏,松脂从松脂管渗漏至周围的管胞中,使水溶性染剂不能着色而出现白斑,树液移动受阻。而对照株中染剂可在植株管胞中自由移动,不会产生白斑,松脂流动只限于松脂管内。而在这段时间接种株和对照株外观上生长正常,并无明显差异。因此,通过酸性品红染色追踪树液流,观察植株根茎横切面白斑的出现可作为早期诊断马尾松松材线虫病的一种方法。
In this study, 3-7-year-old Pinus massoniana seedlings were inoculated with Bursaphelenchus xylophilus, a pine wood nematode isolated from a dead pine tree. The appearance symptoms appeared on the 45th day of the inoculated strain, and the vaccinated plants died within three months. A large number of pine wood nematodes were isolated from the dead plants, indicating that pine wood nematodes have obvious pathogenicity to P. massoniana. In the experiment, the flow of the tree was also followed by acid fuchsine staining. In the early stage of pine wood nematode infection, the parenchyma cells of the pineal tube were destroyed, and the rosin leaked from the rosin to the surrounding tracheids, rendering the water-soluble dye ineffective Coloring and white spots appear, the sap movement blocked. The control plant dye in the plant cells in the free movement, will not produce white, rosin flow is limited to the resin tube. In the meantime, the appearances of inoculated and control strains grew normally, with no significant difference. Therefore, tracking the flow of sap through acid fuchsin staining and observing the occurrence of rhizome cross-section white spots can be used as a method for early diagnosis of P. massoniana nematode disease.