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该研究以培养人体血管平滑肌细胞(VSMC)为模型,用3H-TdR掺入试验观察钙拮抗剂(Nifedipine、Amlodipine)和ACEI(Cilazapril、Benazapril)对细胞增殖效应。结果3种浓度钙拮抗剂都明显抑制细胞增殖(P<0.01),高浓度(10-5mol/L、5×10-6mol/L)时Nifedipine抑制程度更好(P<0.05),低浓度(10-6mol/L)以Amlodipine为佳(P<0.05)。ACEI中发现高浓度时2种药物均能抑制细胞增殖(P<0.01),而低浓度时细胞生长反常地超过对照组(P<0.05)。钙拮抗剂与ACEI比较,前者尤其是Nifedipine抑制细胞增殖程度较ACEI明显。由此推测小剂量ACEI反而刺激细胞增生是否与药物含量较低时仅抑制血浆ACE活性,不能抑制组织ACE活性有关。
In this study, cultured human vascular smooth muscle cells (VSMCs) were used as models to observe the effects of calcium antagonists (Nifedipine, Amlodipine) and ACEI (Cilazapril, Benazapril) on 3H-TdR incorporation. Results All three concentrations of calcium antagonist significantly inhibited cell proliferation (P <0.01). Nifedipine inhibited more effectively at 10-5 mol / L and 5 × 10-6 mol / L (P <0.05) (10-6mol / L) is better than Amlodipine (P <0.05). Both drugs inhibited the cell proliferation (P <0.01) at high concentrations in ACEI, while the cell growth abnormally exceeded those in the control group at low concentration (P <0.05). Calcium antagonist and ACEI compared with the former, especially Nifedipine inhibition of cell proliferation than ACEI obvious. It is inferred that low-dose ACEI but to stimulate cell proliferation and drug content is lower than only inhibit plasma ACE activity, can not inhibit ACE activity.