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用W6/ 3 2单抗对JEG 3细胞进行免疫荧光染色 ,可见在JEG 3细胞膜表面有高强度的黄绿色荧光。HLA G+的JEG 3细胞作为刺激细胞 ,观察其对淋巴细胞增殖反应的影响 ,结果JEG 3细胞不能刺激淋巴细胞增殖。采用经典的单向混合淋巴细胞培养的方法 ,以转染及未转染HLA G分子的K5 62细胞作为抑制细胞 ,按一定的比例加入反应体系 ,结果表明 ,转染HLA G的K5 62细胞能抑制淋巴细胞的增殖反应 ,该抑制以刺激细胞∶反应细胞∶抑制细胞的比例为 1∶1∶2时效果最明显 ,抑制率为 5 4 1% (P <0 0 1) ,转染空质粒和未转染HLA G的K5 62细胞均无明显抑制作用。外周血淋巴细胞与JEG 3细胞共同孵育 ,碘化丙啶 (PI)染色 ,流式细胞仪观察 ,结果发现 ,HLA G分子能诱导淋巴细胞的凋亡。
Immunofluorescence staining of JEG 3 cells with W6 / 3 2 mAb showed a strong yellow-green fluorescence on the surface of JEG 3 cell membrane. JEG 3 cells of HLA G + were used as stimulator cells to observe their effect on lymphocyte proliferation reaction. As a result, JEG 3 cells could not stimulate lymphocyte proliferation. Using classical one-way mixed lymphocyte culture method, K562 cells transfected with non-transfected HLA G molecules as inhibitory cells were added to the reaction system at a certain ratio. The results showed that K562 cells transfected with HLA G Inhibition of lymphocyte proliferation response, the inhibition to stimulate the cells: reaction cells: cell inhibition ratio of 1: 1: 2 most obvious effect, the inhibition rate of 541% (P <0 01), transfected with empty plasmid And not transfected K562 HLA G cells were no significant inhibitory effect. Peripheral blood lymphocytes were incubated with JEG 3 cells, propidium iodide (PI) staining and flow cytometry. The results showed that HLA G molecules could induce lymphocyte apoptosis.