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目的：研究白细胞介素２（ＩＬ－２）、白细胞介素３（ＩＬ－３）基因共转染瘤苗的体内外生物学特性。方法：用ＩＬ－２重组腺病毒（Ａｄ－ＩＬ－２）、ＩＬ－３重组腺病毒（Ａｄ－ＩＬ－３）转染ＦＢＬ－３红白血病细胞株，观察其体外增殖能力及体内致瘤性的变化。结果：ＩＬ－２、ＩＬ－３共转染的ＦＢＬ－３细胞１周内能较稳定地分泌ＩＬ－２和ＩＬ－３，体外增殖能力无明显变化，但体内致瘤性明显下降，ＩＬ－２、ＩＬ－３共转染的ＦＢＬ－３体内致瘤性下降最为显著，肿瘤生长明显延缓，存活期显著延长。结论：ＩＬ－２，ＩＬ－３基因的转入导致ＦＢＬ－３细胞体内致瘤性的下降可能是由于激发体内免疫功能所致。
Objective: To study the in vitro and in vivo biological characteristics of co-transfection of interleukin 2 (IL-2) and interleukin 3 (IL-3) gene in vitro and in vivo. Methods: FBL-3 erythroleukemia cell lines were transfected with recombinant adenovirus (Ad-IL-2) and IL-3 of Ad-IL-3. The proliferation and in vivo tumorigenicity The change. Results: FBL-3 cells co-transfected with IL-2 and IL-3 could stably secrete IL-2 and IL-3 within 1 week, with no significant changes in proliferation in vitro. However, the tumorigenicity of IL- The tumorigenicity of FBL-3 co-transfected with IL-3 in vivo was the most significant, tumor growth was significantly delayed, the survival period was significantly prolonged.2. Conclusion: The decrease of tumorigenicity in FBL-3 cells induced by IL-2 and IL-3 gene transfer may be attributed to the activation of immune function in vivo.