Fingolimod (FTY720) improves postoperative cognitive dysfunction in mice subjected to D-galactose-in

来源 :中国神经再生研究(英文版) | 被引量 : 0次 | 上传用户:flfi2003
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Neurocognitive dysfunction is a common postoperative complication, especially in older adult patients. Fingolimod (FTY720) is a sphin-gosine-1-phosphate receptor modulator that has been found to be neuroprotective in several animal models of central nervous system disease. However, few reports have examined whether FTY720 could mitigate postoperative cognitive dysfunction. In this study, we in-vestigated whether FTY720 could prevent postoperative neurocognitive impairment in mice subjected to D-galactose-induced aging. We induced an accelerated model of aging by administering an intraperitoneal injection of D-galactose. Subsequently, we performed a partial hepatolobectomy under sevoflurane anesthesia. FTY720 (1 mg/kg) was administered intraperitoneally 3 hours before and 24 hours after anesthesia and surgery. Our results indicated that anesthesia and surgery significantly impaired spatial memory in the Y-maze test 6 hours after surgery. We also found that problem solving ability and long-term memory in the puzzle box test on postoperative days 2-4 were significantly improved by FTY720 treatment. Immunohistochemical staining and west blot assay demonstrated that FTY720 signifi-cantly inhibited microglial activation in the hippocampal CA1 region of mice 6 hours and 3 days after anesthesia, and down-regulated the expression of synaptic-related proteins postsynaptic density protein 95 and GluR2 in the hippocampus. These results indicate that FTY720 improved postoperative neurocognitive dysfunction in mice subjected to D-galactose-induced aging. This study was approved by the Ex-perimental Animal Ethics Committee of the Third Xiangya Hospital of Central South University of China (approval No. LLSC (LA) 2016-025) on September 27, 2016.
其他文献
环肺静脉隔离的高功率短时程消融策略正成为心房颤动射频导管消融的新热点.在实现肺静脉电隔离的同时,手术时间 、消融时间以及X线曝光时间显著缩短成为人们采用这一策略的重
2019年12月,新型冠状病毒肺炎( 2019冠状病毒病,coronavirus disease 2019,COVID-19)在武汉爆发,逐渐蔓延至全国.新型冠状病毒肺炎部分患者病情进展迅速,危重患者应尽快行体
肺静脉隔离是治疗心房颤动(简称房颤)的基石,高功率短时程消融作为有望提高肺静脉持久隔离成功率的消融方法,对于有经验的术者,值得尝试和探索,但何为最佳的功率水平 、其是
软测量技术是通过数学模型来估计工程上难以检测的变量值。由于神经网络方法能够描述高度非线性的输入输出关系,因此,基于神经网络的软测量技术已经成为很有吸引力的研究领域,它
目的 探讨消融指数(AI)指导心房颤动(简称房颤)导管消融的短期疗效及安全性.方法 选择2018年6月至2019年5月在苏州大学附属第一医院拟行射频消融术的房颤患者116例为研究对象
3G通信在国内已经发展起来,凭借其自身技术特点和高速的数据传输能力必然给电信业带来影响并掀起重大变革。3G的多媒体数据业务必将取代传统的语音业务成为运营商最重要的利
【目的】孟氏隐唇瓢虫Cryptolaemus montrouzieri Mulsant生殖系统结构和卵细胞发生将为昆虫的系统进化关系及瓢虫分类提供依据,同时可作为瓢虫人工饲料研究开发的参考。【方
Caspase-8 plays an important role in the mediation of inflammation and the effect of its role in subarachnoid hemorrhage remains elu-sive. The nucleotide-bindin
干细胞包括胚胎千细胞(embryonic stem cells,ESCs)和成体T细胞,这类细胞可以在体外培养和快速增殖,同时具有向自我更新和多向分化潜能,是目前多种疾病实验研究和临床治疗的热点
目的 评估高功率短时程(HPSD)消融心房颤动(简称房颤)的安全性.方法 前瞻性观察2019年1月至2019年12月行导管消融的房颤患者,应用HPSD消融方法,评估HPSD方法的术中及术后并发