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建立一种QuEChERS-超高效液相色谱-串联质谱(QuEChERS-UPLC-MS/MS)检测畜禽组织中金刚烷胺残留的方法。样品用1%乙酸乙腈溶液超声提取,提取液加入C18和PSA填料进行基质分散固相净化,浓缩后经Proshell120 EC-C18柱分离,用电喷雾离子源正离子多反应监测(MRM)模式进行检测。在0.2μg/L~100.0μg/L线性范围内具有较好的线性关系,相关系数大于0.997。该方法检出限(LOD)为0.6μg/kg,定量限(LOQ)为2.0μg/kg,回收率为70.5%~100.1%,相对标准偏差(RSD)小于10%。该方法简便快速、准确度好,适用于畜禽组织中金刚烷胺药物残留的检测。
To establish a QuEChERS-UPLC-MS / MS method for the detection of amantadine residues in livestock and poultry tissues. The samples were extracted with 1% acetic acid in acetonitrile. The extracts were added with C18 and PSA fillers for matrix-dispersed solid-phase purification. After concentration, they were separated on a Proshell 120 EC-C18 column and detected by electrospray ionization positive ion multiple reaction monitoring (MRM) . In the linear range of 0.2μg / L ~ 100.0μg / L has a good linear relationship, the correlation coefficient greater than 0.997. The limit of detection (LOD) was 0.6μg / kg, the limit of quantification (LOQ) was 2.0μg / kg, the recovery was 70.5% ~ 100.1% and the relative standard deviation (RSD) was less than 10%. The method is simple, rapid and accurate, and is suitable for the detection of amantadine residues in livestock and poultry tissues.