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目的 观察猝死和非猝死组尸检心脏标本心肌局部Zif/2 68的表达 ,为心肌早期缺血死后诊断提供客观指标。 方法 采用免疫组化SABC法和图像分析与统计学处理系统 ,对 18例猝死和 18例非猝死组尸检心脏标本心肌局部细胞核蛋白Zif/2 68的累积情况进行研究。 结果 18例心性猝死心脏标本心肌局部有部分心肌细胞核呈阳性着色 ,阳性表达率达 10 0 %。 18例对照组标本仅 2例心肌细胞核散在阳性。图像定量分析结果显示两组差异显著。 结论 说明免疫组化染色法检测心肌细胞核Zif/2 68的表达有望成为急性心肌缺血死后诊断的一种有价值有意义的手段。
Objective To observe the expression of Zif / 2 68 in the myocardium of autopsy myocardium in sudden death and non-sudden death group, and to provide an objective index for the diagnosis of early myocardial ischemia. Methods The immunohistochemical SABC method and the image analysis and statistical processing system were used to study the accumulation of myocardial nuclear ribonucleoprotein Zif / 2 68 in 18 cases of sudden death and 18 cases of non-sudden death. Results In 18 cases of sudden cardiac death, cardiac myocytes were partially stained positive in some myocardial nuclei, the positive rate was 100%. 18 cases of control group only 2 cases of cardiomyocyte nuclei scattered in the positive. Image quantitative analysis showed significant differences between the two groups. Conclusions Immunohistochemical staining for the expression of Zif / 2 68 in cardiomyocyte nuclei is expected to be a valuable and meaningful tool for the diagnosis of acute myocardial ischemia.