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以花结抑制试验检测50名妊娠3～8周妇女静脉血混合血清及其经DEAE纤维素离子交换层折和经Sephadex-G-100凝胶过滤后,所得各阶段提取物的花抑滴度,并以HCG胶体金试剂盒测定HCG。对照组为50名处女混合血清,结果证明:混合孕血清经DE-52柱层析后未吸附蛋白部分(DE-I)及Sephadex-G100凝胶过滤后洗脱峰DE-I-GⅡ及DE-I-GⅢ均具花抑活性,DE-I-GⅡ为HCG(+)而DE-I-GⅢ为HCG(-)°提示DE-I-GⅡ峰为EPF与HCG混合物,而DE-I-GⅢ峰为不含HCG而具花抑活性的EPF粗提物。
Antagonistic test was used to determine the antitumor activity of antisera of all three stages of pregnant women with venous blood from 3 to 8 weeks of gestation, DEAE cellulose ion-exchange chromatography and Sephadex-G-100 gel filtration. , And HCG colloidal gold kit determination of HCG. The control group of 50 virgin mixed serum, the results show that: mixed serum after DE-52 column non-adsorbed protein fraction (DE-I) and Sephadex-G100 gel filtration eluting peaks DE-I-G Ⅱ and DE -I-GIII are devoid of activity, DE-I-GII is HCG (+) and DE-I-GIII is HCG (-) °. This suggests that DE-I-GII peak is a mixture of EPF and HCG, The GIII peak is an EPF crude extract that is devoid of HCG and devoid of activity.