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在毛细管超薄池光度检测中首次采用吸收光强测量新技术,显著提高了测量的灵敏度和改善了信噪比.在内径50μm的石英毛细管超薄池以及由石英光导纤维构成的十字交叉超薄光学吸收池上,分别测量了系列高锰酸钾溶液吸收光强.吸收光强与高锰酸钾溶液浓度之间呈现良好的线性关系.对于10μg/mL的高锰酸钾稀溶液,毛细管超薄池吸收光强测量的信噪比相对于吸光度测量有较大的改善.十字交叉池的光程约为40μm,容积约为0.02μL,具有较大的光通量,可望发展成为一种新型的电泳光度检测池.
In the capillary ultrathin pool photometric detection for the first time using absorption light intensity measurement of new technology, significantly increased the measurement sensitivity and improved signal to noise ratio.In the quartz capillary tube with an inner diameter of 50μm ultrathin cell and quartz optical fiber composed of cross-shaped ultra-thin Optical absorbers were measured on a series of potassium permanganate solution absorption intensity of light intensity and potassium permanganate solution showed a good linear relationship between concentration of 10μg / mL potassium permanganate dilute solution, capillary thin The signal-to-noise ratio of the measured absorbance of the pool is greatly improved compared with that of the absorbance measurement.The cross-over cell has an optical path length of about 40 μm and a volume of about 0.02 μL with a large luminous flux and is expected to develop into a new type of electrophoresis Photometric detection pool.