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目的观察柿叶黄酮(PLF)对阿霉素肾病(AN)大鼠尿蛋白、血生化及肾组织免疫病理损伤的影响,初步探讨柿叶黄酮对阿霉素肾病作用机制。方法 70只SD大鼠随机分为7组:正常对照组(A组)、病理对照组(B组)、低中高剂量柿叶黄酮组(C1、C2、C3组)、阳性药物组(D组)及联合用药组(E组)。阿霉素肾病鼠模型建立后,用药组实验鼠分别经胃给予相应药物7周。模型鼠阿霉素(ADR)注射前、注射后及药物干预后测定24 h尿蛋白(24HUP)定量变化,药物干预后测定模型鼠血生化指标,显微镜观察模型鼠肾组织病理形态改变。结果各剂量柿叶黄酮、强的松及联合用药均能明显降低阿霉素肾病鼠24HUP(P<0.01),改善肾脏病理损伤,调节血清蛋白与血脂含量(P<0.05)等。结论柿叶黄酮能降低阿霉素肾病鼠24HUP、调节血生化及改善肾脏病理损伤等,对阿霉素肾病鼠肾脏起到一定的保护作用。
Objective To observe the effects of persimmon leaf flavonoids (PLF) on urinary protein, blood biochemistry and renal pathological changes in rats with adriamycin nephropathy (AN), and to investigate the mechanism of persimmon leaf flavonoids on adriamycin nephropathy. Methods Seventy SD rats were randomly divided into seven groups: normal control group (group A), pathological control group (group B), low, medium and high doses of persimmon leaf flavonoids (group C1, C2 and C3), positive group ) And combination group (group E). After the adriamycin-induced nephropathy mouse model was established, the rats in the drug-treated group were given the corresponding drugs via the stomach respectively for 7 weeks. The 24 h urinary protein (24HUP) was quantified before and after ADR injection, and 24 h urine protein (24HUP) was measured after drug intervention. The blood biochemical indexes of the model mice were determined after the drug intervention, and the pathological changes of the kidney were observed under the microscope. Results All doses of persimmon leaf flavonoids, prednisone and combination therapy could significantly reduce 24HUP of adriamycin-induced nephropathy rats (P <0.01), improve renal pathological injury, regulate serum protein and lipid content (P <0.05). Conclusions Persimmon leaf flavonoids can reduce 24HUP of adriamycin-induced nephropathy rats, regulate blood biochemistry and improve renal pathological injury, and play a protective role in the kidney of adriamycin-induced nephropathy rats.