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利用454高通量测序技术对宁波沿海10个陆源排污口20个站位的放线菌的时空分布及5个工业排污口的放线菌的种类作了整体分析。成功鉴定出了83个属,84个种。研究结果显示:放线菌在陆源排污口的分布呈现季节性分布,从3月份到10月份,放线菌数量呈现先升高后下降的变化,5月份和8月份数量居高,在3月份和10月份偏低;在综合排污口(S4,S6,S8和S9)检测频次较高,在工业排污口(S1,S3,S5,S7和S10)检测频次居中,在市政排污口(S2)检出频次最低。不同类型的排污口,氨氮浓度的排出量不同,放线菌的种类和数量也不同。在5个工业排污口中,S7和S10检出的共同菌最多;在S7独自检出短双歧杆菌(Bifidobacterium breve)、长双歧杆菌(B.longum)和两岐双岐杆菌(B.bifidum),表明存在粪源污染物;在S5检出皱孢链霉菌(Streptomyces scabrisporus)和硫藤黄链霉菌(S.thioluteus),显示有石油降解物和重金属的污染。总体上看,放线菌数量在距排污口外50m处略高于排污口处。排放指标越相似,菌的种类越接近。
Using 454 high-throughput sequencing technique, the temporal and spatial distribution of actinomycetes in 20 stations along the coast of Ningbo and the types of actinomycetes in five industrial sewage outfalls were analyzed. Successfully identified 83 genera and 84 species. The results showed that the distribution of actinomycetes in terrestrial sewage outfalls showed a seasonal distribution. From March to October, the number of actinomycetes increased firstly and then decreased, and the quantity of actinomycetes was the highest in May and August. In March, (S1, S3, S5, S7 and S10), the detection frequency was moderate at the industrial sewage outfalls (S2) The lowest detection frequency. Different types of sewage outfall, ammonia concentration of different discharge, actinomycetes species and quantity are also different. Among the five industrial sewage outfalls, the number of common bacteria detected by S7 and S10 was the highest; in S7, only Bifidobacterium breve, B. longum and B. bifidum ), Indicating the presence of manure contaminants; detection of Streptomyces scabrisporus and S. thioluteus at S5 showed contamination with petroleum degradants and heavy metals. Overall, the number of actinomycetes slightly above the sewage outfall at 50m away from the sewage outfall. The more similar the emission indicators, the closer the species of bacteria.