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该文研究了黄芪多糖(Astragalus polysaccharides,APS)对低氧环境中成骨诱导的骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)成骨分化的作用。BMSCs培养于含40μg/mL APS、氧浓度分别为3%、6%、10%和20%的成骨诱导培养液4周,通过茜素红染色分析BMSCs中骨钙蛋白沉积、Real-time PCR和Western blot分别检测成骨相关基因骨髓基质细胞核心结合因子α1(runtrelated transcription factor 2,Runx2)和骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)的mRNA和蛋白质水平。结果表明,在不同氧浓度下,与单纯成骨诱导剂组相比,含APS的成骨诱导剂组BMSCs中出现了大量桔红色的骨钙蛋白沉积,Runx2和BMP-2的mRNA和蛋白质水平显著升高;尤其在氧浓度为6%时,差异较显著(P<0.05)。40μg/mL APS具有促进低氧环境中成骨诱导的BMSCs成骨分化的作用,其促进作用与氧浓度相关。
This article studies the effect of Astragalus polysaccharides (APS) on osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) induced by hypoxia. BMSCs were cultured in osteogenic medium containing 40μg / mL APS with 3%, 6%, 10% and 20% oxygen for 4 weeks, respectively. Osteocalcin deposition in BMSCs was analyzed by alizarin red staining. Real-time PCR And Western blot were used to detect the mRNA and protein levels of osteoblast-related genes, bone marrow stromal cell core-associated factor α1 (rux-2) and bone morphogenetic protein-2 (BMP-2) The results showed that a large number of orange-red osteocalcin deposits were found in BMSCs with different levels of osteoblast-inducing agent and osteoblast-inducing agents at different oxygen concentrations. The mRNA and protein levels of Runx2 and BMP-2 (P <0.05), especially at oxygen concentration of 6%. 40μg / mL APS can promote the osteogenic differentiation of BMSCs induced by osteogenesis in hypoxia environment, and its promotion is related to the oxygen concentration.