论文部分内容阅读
[目的]观察海洋胶原肽对四氧嘧啶诱导糖尿病大鼠血清脂质和抗氧化水平的影响。[方法]通过对SD大鼠腹腔注射四氧嘧啶建立糖尿病大鼠模型,随机分为海洋胶原肽低、中、高剂量组和高糖模型对照组,分别灌胃给予海洋胶原肽0.225g/kg.BW、0.450g/kg.BW、1.35g/kg.BW和同体积蒸馏水;另设定正常高剂量组和正常空白对照组,灌胃给予1.35g/kg.BW海洋胶原肽和同体积蒸馏水,连续8周。取大鼠尾血,测量血清脂质(TG、TC、LDL-C、HDL-C)和抗氧化指标(MDA、SOD)。[结果]试验开始时(0周)海洋胶原肽各剂量组大鼠TC、TG、MDA水平明显高于正常空白对照组,HDL-C、SOD水平则明显较低(P﹤0.05);与高糖模型对照组相比差异无统计学意义。干预8周后海洋胶原肽各剂量组血清TC水平仍高于正常空白对照组(P﹤0.05);而TG水平与正常对照组差异无统计学意义(P﹥0.05);4周末时低、高剂量组HDL-C明显上升,甚至高于正常空白对照组(P﹤0.05);LDL-C试验前后组间均无明显差异;各剂量组SOD活力仍明显低于正常空白对照组(P﹤0.05);MDA水平高于正常对照组,但低于高糖对照组(P﹤0.05)。[结论]海洋胶原肽可有效改善四氧嘧啶诱导糖尿病大鼠的脂代谢紊乱,并有一定的抗氧化损伤功能;由于糖尿病大鼠受损较严重,以上结论有待进一步研究证实。
[Objective] To observe the effect of marine collagen peptide on serum lipid and anti-oxidation in alloxan-induced diabetic rats. [Method] The model of diabetic rats was established by intraperitoneal injection of alloxan into SD rats. The model rats were randomly divided into low, medium and high doses of marine collagen peptide group and high glucose model control group. The rats were given 0.225 g / kg .BW, 0.450g / kg.BW, 1.35g / kg.BW and the same volume of distilled water; another set of normal high-dose group and normal blank control group, intragastric administration of 1.35g / kg.BW marine collagen peptide and the same volume of distilled water For 8 weeks. Serum lipids (TG, TC, LDL-C, HDL-C) and antioxidant index (MDA, SOD) were measured in rat tail blood. [Results] At the beginning of the experiment (0 week), the levels of TC, TG and MDA in the marine collagen peptide groups were significantly higher than those in the normal control group, while the HDL-C and SOD levels were significantly lower (P <0.05) There was no significant difference between sugar model and control group. After 8 weeks of intervention, the level of TC in each dose group of marine collagen peptide was still higher than that of the normal control group (P <0.05), but there was no significant difference between TG level and normal control group (P> 0.05) HDL-C in the dose group increased significantly, even higher than that in the normal control group (P <0.05). There was no significant difference between the two groups before and after the LDL-C test. The SOD activity in each dose group was still significantly lower than that in the normal control group ); MDA level was higher than the normal control group, but lower than the high glucose control group (P <0.05). [Conclusion] Marine collagen peptide can effectively improve alloxan-induced diabetic rats lipid metabolism disorders, and have some anti-oxidative damage; due to the more serious damage in diabetic rats, the above conclusion remains to be confirmed by further studies.