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目的观察青心酮防治ApoE(-/-)小鼠主动脉粥样硬化病变(atherosclerosis,AS)与5-脂氧合酶的关系。方法传代培养小鼠巨噬细胞株RAW264.7,分成4组:正常对照组,模型组,青心酮组,辛伐他汀组。收集各组细胞蛋白,应用Western-blot检测5-脂氧合酶含量;收集上清液,用ELISA检测白三烯B4(leukotrieneB4,LTB4)。以C57BL/6小鼠作正常对照组,24只雄性ApoE(-/-)小鼠随机分成3组:模型组(等量乙醇),青心酮治疗组(20 mg·kg-1·d-)1,辛伐他汀治疗组(20 mg·kg-1·d-)1,给药8周。所有实验小鼠饲以普通饲料至16周。取血检测LTB4,剪取主动脉根部行组织切片,HE染色观察主动脉粥样硬化病变情况;剪取主动脉根部斑块组织,Western blot法测定斑块中5-脂氧合酶水平。结果青心酮处理后LTB4降低,5-脂氧合酶含量减少,AS病灶形成数目、面积减少。结论青心酮能减轻ApoE(-/-)小鼠动脉粥样硬化病变,可能与其抑制5-脂氧合酶,减少LTB4有关。
OBJECTIVE To observe the effect of abamectone on the prevention and treatment of apoE (- / -) mouse aorta atherosclerosis (AS) and 5-lipoxygenase. Methods The murine macrophage cell line RAW264.7 was subcultured and divided into 4 groups: normal control group, model group, macropinone group and simvastatin group. The protein of each group was collected, and the content of 5-lipoxygenase was detected by Western-blot. The supernatant was collected and leukotriene B4 (LTB4) was detected by ELISA. Twenty-four male ApoE (- / -) mice were randomly divided into 3 groups: C57BL / 6 mice (normal group), model group ) 1, simvastatin treatment group (20 mg · kg-1 · d-) 1, 8 weeks. All experimental mice were fed a normal diet until 16 weeks. Blood samples were taken for detection of LTB4, the aortic roots were cut for histological sections, and the aortic atherosclerosis lesion was observed by HE staining. The aortic root plaque was cut out and the level of 5-lipoxygenase in the plaques was determined by Western blot. Results After treatment of Qingxin ketone, LTB4 decreased, the content of 5-lipoxygenase decreased, the number of AS lesions formed and the area decreased. Conclusions Qingxin ketone can attenuate the atherosclerotic lesions in ApoE (- / -) mice, which may be related to the inhibition of 5-lipoxygenase and the reduction of LTB4.