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目的建立钩吻二氯甲烷部位的化学成分指纹图谱。方法采用RP-HPLC方法,依利特色谱柱(250 mm×4.6mm,5μm);流动相A为乙腈,流动相B为水(含0.3%磷酸-0.5%三乙胺);梯度洗脱;流速1 ml·min-1;柱温40℃;进样体积20μl;检测波长254 nm。并对钩吻二氯甲烷部位进行指纹图谱定性分析。结果 10份不同产地钩吻二氯甲烷部位的指纹图谱经《中药色谱指纹图谱相似度评价系统(2004A版)》分析相似度较高,共检出9个相对位置稳定的共有峰,通过与标准品的保留时间及紫外光谱比较,可鉴定为钩吻碱甲和钩吻碱丙,保留时间分别为17.18 min和32.52 min。结论钩吻药材二氯甲烷部位指纹图谱的建立为药材质量控制提供了科学依据。
Objective To establish a fingerprinting chemical composition fingernail CH2Cl2. Methods The RP-HPLC method was used, and the elution column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase A was acetonitrile and the mobile phase B was water (containing 0.3% phosphoric acid and 0.5% triethylamine) 1 ml · min-1; column temperature 40 ℃; injection volume 20μl; detection wavelength 254 nm. The fingerprints of methylene chloride were qualitatively analyzed by fingerprinting. Results Fingerprints of 10 methylene chloride samples of different origins were analyzed by “similarity evaluation system of chromatographic fingerprints of traditional Chinese medicines (2004A version)”, and 9 common peaks with relative stable positions were detected. The retention time and UV spectra of the products were identified as nicotine and kainikin, with retention times of 17.18 min and 32.52 min, respectively. Conclusion The establishment of fingerprints of Radix kaempferiae in dichloromethane can provide a scientific basis for the quality control of medicinal materials.