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建立了OPA柱前衍生-HPLC检测豆制品和发酵糙米中γ-氨基丁酸的方法。样品使用2%三氯乙酸超声提取,经邻苯二甲醛衍生,默克RP C18柱分析(250 mm×4.6 mm,5μm),流动相-乙腈︰0.02 mol/L醋酸钠缓冲液=23︰77,洗脱,检测波长为333 nm。该方法线性范围2.50~250μg/mL,R2=0.999 7。重现性相对标准偏差(n=6)为3.78%,回收率89.3%~107.5%,检出限10.0mg/kg。此方法与传统的分光光度法相比适用性更强,并能和17种氨基酸做到基线分离。
A method for the determination of γ-aminobutyric acid in soy products and fermented brown rice by OPA pre-column derivatization-HPLC was established. The samples were extracted with 2% trichloroacetic acid by ultrasonic and analyzed by RP-C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile: 0.02 mol / L sodium acetate buffer solution = 23: 77 , Elution, detection wavelength of 333 nm. The linear range of this method was 2.50 ~ 250μg / mL, R2 = 0.999 7. The relative standard deviation of reproducibility (n = 6) was 3.78%, the recovery was 89.3% ~ 107.5%, the detection limit was 10.0mg / kg. This method is more applicable than the traditional spectrophotometry, and can be baseline and 17 kinds of amino acids isolated.