蛹草(Cordyceps militaris),也称北冬虫夏草,其药性与传统中药材冬虫夏草(C.sinensis)相近。我们在罐头瓶内多次育成蛹草子座,现将方法介绍如下。 (一)培养基的制作与接种方法普通大米:高梁米=9:1,使含水量达到110-120％,其中含KH_2PO_40.1％,MgSO_4 0.05％,酵母粉0.5％,蛋白质少量。每瓶加入50-100克干重的培养基。灭菌时间:高压1.5-2小时,常压8-10小时。取斜面菌种一小片或米饭培养基菌种一小块,在无菌条件下接种在罐头瓶内。菌种特点:在斜面培养基上菌丝纯白色、粗壮浓密,紧贴培养基生长,边缘清楚;在米饭培养基上容易形成浅桔黄色的色素沉着。 (二)栽培管理菌株接种后,在15℃左右条件下培养。6-10℃生长缓慢,20-25℃有利于子座形成。培养基含水量低于100％以下,生长缓慢;湿度过 Cordyceps militaris, also known as Cordyceps militaris, is similar in potency to C. sinensis, a traditional Chinese herbal medicine. We repeatedly raise pupa turkey in canned bottles, the method is as follows. (A) medium preparation and inoculation method ordinary rice: sorghum rice = 9: 1, so that the water content of 110-120%, which contains KH_2PO_40.1%, MgSO_4 0.05%, yeast 0.5%, a small amount of protein. Add 50-100 g dry weight of media per bottle. Sterilization time: high pressure 1.5-2 hours, atmospheric pressure 8-10 hours. Take a small piece of bevel bacteria or rice culture medium a small piece, inoculated under sterile conditions in a canned bottle. Strain characteristics: mycelium in slant medium pure white, thick dense, close to the growth of the medium, the edge clear; easy to form light orange yellow pigmentation in rice medium. (B) cultivation and management strains inoculated, cultured at about 15 ℃ conditions. 6-10 ℃ slow growth, 20-25 ℃ is conducive to the formation of the child seat. Medium moisture content below 100%, slow growth; humidity too