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为探讨NO对渗透胁迫下茶树(Camellia sinensis(L.)O.Kuntze)种子萌发的影响,用20%PEG6000模拟干旱处理,分别添加0、20、50、100、200和300μmol·L~(-1)硝普钠(SNP,NO外源供体)。结果表明,外源NO能显著促进渗透胁迫下茶树种子的萌发,且呈现一定的浓度效应,在NO供体SNP浓度为100μmol·L~(-1)时效果最佳。随着外源SNP浓度的增加,茶树种子的萌发及生长受到了抑制,种子的发芽率、发芽指数、根长和活力指数均呈降低的趋势。进一步研究表明100μmol·L~(-1)的SNP能促进SOD、POD、CAT、APX、GR和GPX等抗氧化酶活性的提高,降低MDA含量及相对电导率。推测添加外源NO后,抗氧化酶活性提高,降低了茶树种子的氧化损伤,从而缓解了渗透胁迫对茶树种子萌发的抑制作用。
In order to investigate the effect of NO on the seed germination of tea plant (Camellia sinensis (L.) O.Kuntze) under osmotic stress, 20% PEG6000 was used to simulate the drought treatment, and 0, 20, 50, 100, 200 and 300μmol·L ~ 1) Sodium nitroprusside (SNP, NO exogenous donor). The results showed that exogenous NO could significantly promote the germination of tea seed under osmotic stress and showed a certain concentration effect, which was the best when NO donor SNP concentration was 100μmol·L -1. With the increase of exogenous SNP concentration, the germination and growth of tea seed were inhibited. The seed germination rate, germination index, root length and vigor index all tended to decrease. Further studies showed that 100μmol·L -1 SNP could promote the activities of antioxidant enzymes such as SOD, POD, CAT, APX, GR and GPX and decrease the content of MDA and the relative conductivity. It is speculated that adding exogenous NO enhanced the activity of antioxidant enzyme and reduced the oxidative damage of tea seed, thus alleviating the inhibitory effect of osmotic stress on the germination of tea seed.