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在0.5mmol·L-1Cd2+处理时,小五缨(XWY)的发芽率下降至92%,并且随着Cd2+浓度的增加,发芽率逐渐降低,在1和5mmol·L-1Cd2+浓度处理时,发芽率分别降至83%和67%。象牙白(XYB)则在5mmol·L-1Cd2+浓度时,发芽率下降。卫青(WQ)在0.05mmol·L-1Cd2+浓度时,发芽率已降至83%,1和5mmol·L-1时则下降至58%。幼苗生长也明显受Cd2+处理影响,在0.05mmol·L-1Cd2+处理时,3种栽培萝卜的幼苗生长均受到明显影响,并且随Cd2+浓度的增加,生长受抑加重。从发芽率和幼苗生长两种实验结果看,卫青对Cd2+最为敏感。双向电泳结果表明,Cd2+处理后3种栽培萝卜幼苗中蛋白质组分有明显变化。小五缨中,0.1mmol·L-1Cd2+处理后,有5个蛋白质点消失,15个新的蛋白质点被诱导产生。象牙白中,2个蛋白质点消失,1个蛋白质点含量减少,13个新的蛋白质点被诱导产生。卫青中,12个新的蛋白质点诱导产生,但没有发现蛋白质点消失现象。Cd2+处理后,3种栽培萝卜中,蛋白质合成的变化与幼苗生长受抑存在明显相关性,这一实验结果对于探讨萝卜Cd2+害的生化机理是有重要意义的。
The germination rate of XWY decreased to 92% at 0.5 mmol·L-1Cd2 + treatment, and the germination rate gradually decreased with the increase of Cd2 + concentration. When the treatments were at 1 and 5 mmol·L-1Cd2 + concentrations, The rates dropped to 83% and 67% respectively. Ivory (XYB) in the 5mmol · L-1Cd2 + concentration, the germination rate decreased. At the concentration of 0.05mmol·L-1 Cd2 +, the germination rate of WQ decreased to 83% and decreased to 58% at 1 and 5mmol·L-1. Seedling growth was also significantly affected by Cd2 + treatment. Under 0.05mmol·L-1Cd2 + treatment, the seedling growth of three radish cultivars were significantly affected, and the growth inhibition was aggravated with the increase of Cd2 + concentration. From the germination rate and seedling growth of two experimental results, Wei Qing is the most sensitive to Cd2. Two-dimensional electrophoresis results showed that Cd2 + treatment of three cultivated radish seedlings in the protein composition has significantly changed. Small five Ying, 0.1mmol·L-1Cd2 + treatment, five protein spots disappear, 15 new protein spots were induced. Ivory, two protein spots disappear, a protein content decreased, 13 new protein spots are induced. Weiqing, 12 new protein spots were induced, but no protein spots disappeared. Cd2 + treatment, the three kinds of cultivated radish, the changes in protein synthesis and seedling growth inhibition was significantly correlated, the experimental results for the study on the biochemical mechanism of radish Cd2 + is of great significance.