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目的:探讨大鼠尾端延髓腹外侧区(CVLM)非N-甲基-D-天门冬氨酸(non-NMDA)受体在介导压力反射中的作用。方法:在戊巴比妥钠和氨基甲酸乙酯麻醉、制动和人工呼吸的SD大鼠,观察CVLM内局部给予non-NMDA受体选择性阻断剂CNQX对刺激主动脉神经导致的减压反应和头端延髓腹外侧区(RVLM)神经元的压力敏感性的作用。结果:双侧CVLM微量注射CNQX(每侧200 pmol/100 nL)后明显(P<0.01)地增高基础血压(BP)和心率,而且显著(P<0.01)地减弱刺激主动脉神经导致的降压反应。单侧CVLM内给予CNQX(200 pmol/100 nL)明显(P<0.01)增高同侧RVLM压力敏感性神经元的基础放电频率,但显著(P<0.01)减弱刺激主动脉神经和升高血压导致的神经元活动的抑制效应和部分抑制神经元的心性节律。结论:大鼠尾端延髓腹外侧区non-NMDA受体在维持紧张性兴奋的心血管活动和传递压力感受器信息中具有重要的意义。
AIM: To investigate the role of non-N-methyl-D-aspartate (NMDAR) receptors in the medullary ventrolateral medulla (CVLM) in the mediation of stress reflex in rats. METHODS: SD rats, anesthetized with sodium pentobarbitol and urethane anesthesia, braked and resuscitated, were treated with CNQX, a selective blocker of non-NMDA receptor, locally administered to CVLM to induce decompression of aortic nerves Response and pressure sensitivity of the rostral ventrolateral medulla (RVLM) neurons. RESULTS: After bilateral microinjection of CNQX into bilateral CVLMs (200 pmol / 100 nL on each side), basal BP and heart rate were significantly increased (P <0.01) and significantly decreased (P <0.01) Pressure reaction. CNQX (200 pmol / 100 nL) in unilateral CVLM significantly (P <0.01) increased the basal discharge frequency of ipsilateral RVLM pressure-sensitive neurons, but significantly (P <0.01) attenuated the stimulation of aortic nerves and increased blood pressure Inhibition of neuronal activity and partial inhibition of neuronal rhythms. CONCLUSION: The non-NMDA receptor in the rostral ventrolateral medulla oblongata plays an important role in maintaining cardiovascular activity and transmitting baroreceptor information of stress.