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目的建立以HPLC法测定六味木香胶囊中木香烃内酯和去氢木香内酯的方法。方法色谱柱为VP-ODS柱(250mm×4.6 mm,5μm),流动相为甲醇-水(72∶28),检测波长为225 nm,柱温为25℃,体积流量为1.0 mL/min。结果木香烃内酯、去氢木香内酯进样量分别在203.12~2 031.20、207.84~2 078.40 ng与峰面积积分值呈良好的线性关系(r=0.999 9);平均回收率分别为101.1%、101.2%,RSD分别为0.48%、0.73%(n=6)。结论建立的方法简便、准确、重复性好、专属性强,可用于该制剂的质量控制和评价。
OBJECTIVE To establish a HPLC method for the determination of carotone lactone and dehydrocostus lactone in Liuwei Juxiang Capsules. Methods The column was VP-ODS (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (72:28). The detection wavelength was 225 nm. The column temperature was 25 ℃ and the volume flow rate was 1.0 mL / min. The results showed that the injection volume of wood aldehyde lactone and deshydrocostus lactone showed a good linear relationship (203.92 ~ 2.031.20, 207.84 ~ 2078.40 ng) with the peak area integral value (r = 0.999 9). The average recoveries were 101.1% and 101.2% with RSDs of 0.48% and 0.73%, respectively (n = 6). Conclusion The method is simple, accurate, reproducible and specific. It can be used for the quality control and evaluation of the preparation.