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目的:建立同时测定千里光药材中对羟基苯甲酸和邻羟基苯甲酸总含量及对羟基苯乙酸含量的方法。方法:采用HPLC法,色谱柱:Shiseido(Fine Chemicals)Capcell Pak C18柱(250 mm×4.6 mm,5μm);流动相:甲醇∶水∶甲酸(13∶87∶0.5);流速:1.0 ml·min-1;检测波长:240 nm;柱温为室温。结果:对羟基苯甲酸和邻羟基苯甲酸的总浓度在0.025~0.400 mg·ml-1范围内线性关系良好(r=0.999 8),平均加样回收率为97.59%,RSD=1.22%(n=6);对羟基苯乙酸的浓度在0.05~0.80mg·ml-1范围内线性关系良好(r=0.999 8),平均加样回收率为98.07%,RSD=1.90%(n=6)。结论:该方法操作简单,重现性好,适用于千里光中对羟基苯甲酸、邻羟基苯甲酸和对羟基苯乙酸的含量测定。
OBJECTIVE: To establish a method for the simultaneous determination of p-hydroxybenzoic acid and o-hydroxybenzoic acid and p-hydroxybenzene acetic acid in Senri Optus. Methods: The HPLC method was adopted. The column was Shiseido (Fine Chemicals) Capcell Pak C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol: water: formic acid -1; detection wavelength: 240 nm; column temperature is room temperature. Results: The total concentration of p-hydroxybenzoic acid and o-hydroxybenzoic acid was good (r = 0.999 8) in the range of 0.025-0.400 mg · ml-1 with an average recovery of 97.59% and a RSD of 1.22% (n = 6). The linearity of p-hydroxyphenylacetic acid in the range of 0.05-0.80 mg · ml-1 was good (r = 0.999 8). The average recoveries were 98.07% and RSD = 1.90% (n = 6). Conclusion: The method is simple and reproducible. It is suitable for the determination of p-hydroxybenzoic acid, o-hydroxybenzoic acid and p-hydroxyphenylacetic acid in Senecio.