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根据表达重组人生长激素的大肠杆菌的自身特点 ,对其发酵的工艺和条件作了初步的研究和改进 ,通过以甘油代替葡萄糖作为培养基中的碳源 ,以流加的方式在发酵过程中逐步补加 ;同时 ,通过控制搅拌转速 ,以控制溶氧不低于 2 0 %的水平 ,在发酵的中后期 ,再以流加的方式补充适量的蛋白胨等营养成份 ,使发酵终止时 ,大肠杆菌的密度由 30 g/L提高到 90 g/L,而 r- h GH的细胞表达量并未因此而受到影响 ,发酵周期从 16 h缩短到 10 h。
Based on the characteristics of Escherichia coli expressing recombinant human growth hormone, the fermentation process and conditions were preliminarily researched and improved. By using glycerol instead of glucose as the carbon source in the culture medium, At the same time, by controlling the mixing speed to control the dissolved oxygen level of not less than 20%, in the latter part of the fermentation, and then by way of adding additional amount of peptone and other nutrients, the termination of fermentation, the large intestine The density of Bacillus increased from 30 g / L to 90 g / L, but the cell number of r-h GH was not affected. The fermentation cycle was shortened from 16 h to 10 h.