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作者以往已证实培养的角朊细胞释放到培养基中的表皮细胞衍生的胸腺细胞活化因子(ETAF)是一种细胞激活因子,其生物学、生物化学以及分子生物学特性与 IL-1相似或相同,并证实了新生儿的角朊细胞决定着他的培养基对其他的新生儿角朊细胞具有生长调节作用。为了检查 ETAF 的自体分泌生长刺激的可能性和供体年龄对这一过程的影响,作者将外源性 ETAF 加到取自新生儿或成人供体的角朊细胞培养物中,分别测定新生儿和成人培养物中 ETAF 的产量,进一步探索评价 ETAF 在角朊细胞生长中的作用。实验包括利用改良Rheinwald-Green 技术,进行新生儿包皮和健康成人上臂内侧皮肤的角朊细胞培养;培养了角朊细胞后的培养基(K-CM)中 ETAF 的生物学测定和 ETAF 的制备3个部分。
The authors have previously demonstrated that epidermal cell-derived thymocyte activation factor (ETAF) released by cultured keratinocytes into the culture medium is a cell-activating factor with similar biological, biochemical and molecular biological properties as IL-1 or The same, and confirmed that newborn keratinocytes determine his medium to other neonatal keratinocytes have a growth-regulating role. To examine the potential of autologous secretory growth stimulation of ETAF and the effects of donor age on this process, the authors added exogenous ETAF to keratinocyte cultures taken from neonates or adult donors and measured neonatal And ETAF production in adult cultures to further explore the role of ETAF in keratinocyte growth. Experiments included keratinocyte cultures of the medial upper arm of neonates with circumcised and healthy adults using modified Rheinwald-Green technology; biological assays of ETAF and preparation of ETAF in cultured keratinocyte-conditioned medium (K-CM) 3 Part.