论文部分内容阅读
作者向小鼠股四头肌注射流感病毒A/PR/8/34株的血凝素(HA)DNA10ng～100μg,共1～3次,每次间隔3周.用血凝抑制(HI)试验和ELISA检测免疫后小鼠体内抗HA抗体水平.两项试验结果都显示HA DNA注射剂量和注射次数与产生的抗体水平直接相关.注射lμg HA DNA三次或一次注射10～100μg HA DNA的小鼠体内均可测到抗体,而100μg HA DNA诱导的抗体水平至少与感染活流感病毒(A/PR/8/34)后产生的抗体水平相仿.免疫后抗体水平至少可维持18个月.注射次数对抗体水平影响不大.
The authors injected 10 ng to 100 μg of hemagglutinin (HA) DNA of influenza A / PR / 8/34 strain into the quadriceps of mice for 1 to 3 times at intervals of 3 weeks, using the hemagglutination inhibition (HI) And ELISA to detect the level of anti-HA antibody in mice after the immunization.The results of both tests showed that HA DNA injection dose and the number of injections directly related to the level of antibody produced.A mouse injected with lμg HA DNA three times or once with 10 ~ 100μg HA DNA Antibodies can be detected in vivo, whereas the level of antibody induced by 100 μg HA DNA is at least as high as that produced after infection with live influenza virus (A / PR / 8/34). The antibody level after immunization is maintained for at least 18 months. Antibody level has little effect.