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为了研究季节性流感裂解疫苗在小鼠中针对甲型流感病毒同型同株、同型异株、异型异株攻击的免疫保护效力及其与诱发的血凝抑制(HI)抗体滴度的关系,本研究使用我国2008~2009年度季节性流感裂解疫苗中不同剂量的甲1型流感病毒H1N1(疫苗株病毒A/Brisbane/59/2007(H1N1)-like)和甲3型流感病毒的H3N2(疫苗株病毒A/Brisbane/10/2007(H3N2)-like)疫苗组分免疫BALB/c小鼠,首先确定了能在小鼠中诱发血HI抗体滴度达到40的疫苗免疫剂量;然后以此剂量免疫小鼠,分别使用同型同株流感病毒(鼠肺适应株A/Brisbane/59/2007(H1N1)-like virus(MA))(简称A1)和同型异株流感病毒(鼠肺适应株A/Purto Rico/8/34(H1N1))(简称PR8)攻击H1N1疫苗免疫小鼠,使用异型异株流感病毒A1攻击H3N2疫苗免疫小鼠,通过体重变化和存活率情况,探讨季节性流感疫苗在小鼠中针对甲型流感病毒同型同株、同型异株、异型异株攻击的保护效力。结果显示,季节性流感裂解疫苗H1N1和H3N2组分按照HA不同剂量0.15μg、0.5μg、1.5μg、5μg和15μg免疫小鼠后,所诱发的HI抗体滴度随免疫剂量的增加而增强,1.5μgHA即可以诱发免疫小鼠HI抗体滴度达到40;以此剂量免疫小鼠,分别使用3LD50、10LD50、30LD50、100LD50、300LD50、1 000LD50和3 000LD50的同型同株流感病毒A1进行攻击,1.5μgH1N1疫苗可以100%保护小鼠抵御高至1000LD50同型同株流感病毒A1的攻击,15μg甚至可以100%保护3 000LD50同型同株流感病毒A1的攻击,但是这两个剂量免疫的小鼠在低至3LD50同型异株流感病毒PR8的攻击后都全部死亡;使用可以诱发HI抗体滴度达到140的15μg H3N2疫苗免疫小鼠,在低至3LD50异型异株流感病毒A1的攻击后亦全部死亡。以上结果表明,季节性流感疫苗可使小鼠HI抗体滴度达到40的疫苗免疫剂量为1.5μg,该免疫剂量可以有效保护小鼠抵御同型同株流感病毒的攻击,但是难以保护小鼠抵御同型异株与异型异株流感病毒的攻击,这一结果为建立以季节性流感疫苗为参考的免疫保护评价体系提供了实验依据。
The objective of this study was to investigate the protective efficacy of seasonal flu-splitting vaccines against the isotype, homozygous, allogeneic challenge and their association with induced hemagglutination inhibition (HI) antibody titer in mice In this study, we investigated the effects of H1N1 (vaccine strain A / Brisbane / 59/2007 (H1N1) -like) and H3N2 (influenza vaccine strain H3 Vaccine components of BALB / c mice were immunized with the virus A / Brisbane / 10/2007 (H3N2) -like vaccine component. First, the vaccine dose that induced the titer of the HI antibody to reach 40 in mice was determined. Mice were infected with the same type of influenza virus (A / Brisbane / 59/2007 (H1N1) -like virus (MA)) (A1) and the same type of influenza virus (A / Purto Rico / 8/34 (H1N1)) (PR8) were challenged with the H1N1 vaccine to challenge the mice with the heterologous influenza A virus H3N2 vaccine. The body weight and survival rate were used to investigate the effects of seasonal influenza vaccine in mice In the same type of influenza A virus with the same type, the same type of plant, heterotypic heterologous attack the protective effect force. The results showed that the titer of HI antibody induced by H1N1 and H3N2 components of mice immunized with 0.15μg, 0.5μg, 1.5μg, 5μg and 15μg of HA was significantly increased with the dose of 1.5 μg HA to induce immunization of mice with HI antibody titers of 40; mice were immunized at this dose with 3LD50, 10LD50, 30LD50, 100LD50, 300LD50, 1 000 LD50 and 3 000 LD50 of the same strain of the same strain of influenza virus A1 challenged with 1.5 μg of H1N1 The vaccine protected 100% of mice against attacks of up to 1000 LD50 of the same-type influenza A virus and 15 μg protected even 100 000% of the homologs of the same strain of influenza A 1 virus, but both doses of the vaccine immunized mice down to 3 LD50 All challenged with the homologous influenza virus PR8 all died; mice immunized with a 15 μg H3N2 vaccine that induced HI antibody titers of 140 all died after challenge down to 3LD50 ALL virus. The above results showed that the vaccine dose of 1.5μg could achieve a titer of HI antibody of 40h when the seasonal influenza vaccine was administered, which could effectively protect mice against the homologous influenza virus, but it was difficult to protect the mice against the isotype Heterologous and heterotypic influenza virus attack, the results provide an experimental basis for the establishment of an immune protection evaluation system based on seasonal influenza vaccine.