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本文用放射受体及放射免疫分析法分别测定脑池脑脊液中内啡肽总量及脑啡肽含量来研究内啡肽释放是否亦表现在脊髓上水平。第一部分实验20只家兔,均分为对照、手针足三里、电针足三里及电刺激脑中央灰质4组。手针、电针及电刺激脑后在局麻中穿刺脑池吸取脑脊液1毫升,用 SephadexG-10柱层析去盐,盐峰前洗脱液合并成组分Ⅰ,盐峰后洗脱液合并成组分Ⅱ,后者与已知脑啡肽洗脱部位相同。用放射受体法测吗啡样活性,手针、电针及电刺激脑组的组分Ⅰ平均值比对照组分别增加22%、31%和20%,组分Ⅱ分别增加10%、15%和2%,但数据较离散均无统计显著意义。第二部分实验家兔15只,均分为对照、脑室内注射杆菌肽50微克及脑室内注射杆菌肽50微克+手针足三里3组。同法抽取脑脊液用放射免疫法测定脑啡肽含量。结果单注射杆菌肽不增加脑啡肽含量,但杆菌肽+手针组增加十分显著,为对照组的3倍。说明静息状态下脑啡肽神经原活动程度较低,针刺可使它大大激活。
In this paper, radioactive receptor and radioimmunoassay were measured cerebrospinal fluid cerebrospinal fluid total enkephalin and enkephalin content to study the endorphin release is also shown in the spinal cord level. The first part of the experiment 20 rabbits were divided into control, hand acupuncture Zusanli, electroacupuncture at Zusanli and electrical stimulation of cerebral gray matter in four groups. Hand acupuncture, electroacupuncture and electro-acupuncture in the brain after anesthesia in the cerebrospinal fluid puncture 1 ml of cerebrospinal fluid, using SephadexG-10 column chromatography desalination, salt peak eluent before merged into component I, salt peak eluate Merged into component II, which is identical to the known enkephalins elution site. The morphine-like activity was measured by radioimmunoassay. The average value of component I in acupuncture, electroacupuncture and electro-acupuncture groups increased by 22%, 31% and 20% respectively compared with that in control group. The percentages of component II increased by 10% and 15% And 2% respectively, but the data were not statistically significant. The second part of the experimental rabbit 15, were divided into control, intraventricular injection of bacitracin 50 micrograms and intraventricular injection of bacitracin 50 micrograms + hands Zusanli 3 groups. Cerebrospinal fluid was extracted by the same method and the enkephalin content was determined by radioimmunoassay. Results Single injection of bacitracin did not increase the content of enkephalin, but the bacitracin + hand acupuncture group increased significantly, three times as much as the control group. Resting state neuropeptide neuropeptide activity is low, acupuncture can make it greatly activated.