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目的:观察TGF-β1对细粒棘球蚴体外与BALB/c小鼠T淋巴细胞培养的影响。方法:阻断剂组:BALB/c小鼠脾细胞+细粒棘球蚴+SB525334共培养;对照组:BALB/c小鼠脾细胞+细粒棘球蚴+PBS共培养;空白对照:BALB/c小鼠脾细胞+RPMI-1640培养基+SB525334共培养。方法:48 h收集淋巴细胞,流式细胞仪检测T淋巴细胞亚群、CD4+CD25+T细胞的数量、NK细胞的数量及其活性受体NKG2D的表达;乳酸脱氢酶法检测NK细胞的杀伤活性。结果:体外阻断TGF-β1受体后,引起细粒棘球蚴介导的CD4+T细胞数量升高、CD8+T细胞数量降低、CD4+/CD8+T细胞比值升高、CD4+CD25+T细胞数量减少、NK细胞活性受体NKG2D的表达增加、NK细胞对靶细胞Yac-1的裂解率增加和NK细胞活性及其活性受体NKG2D成正相关。结论:体外阻断TGF-β1受体可提高T淋巴细胞免疫,从而抵制细粒棘球蚴的感染。
Objective: To observe the effect of TGF-β1 on the culture of Echinococcus granulosus in vitro and in BALB / c mouse T lymphocytes. BALB / c mice splenocytes + Echinococcus granulosus + SB525334 co-culture; control group: BALB / c mice splenocytes + Echinococcus granulosus + PBS co-culture; blank control: BALB / c mouse spleen cells + RPMI-1640 medium + SB525334. Methods: Lymphocytes were harvested at 48 hours. The number of T lymphocyte subsets, CD4 + CD25 + T cells, the number of NK cells and the expression of NKG2D were detected by flow cytometry. Lactate dehydrogenase Killing activity. Results: After blocking TGF-β1 receptor in vitro, the number of CD4 + T cells, the number of CD8 + T cells, the ratio of CD4 + / CD8 + T cells and CD4 + CD25 + The number of T cells was decreased, the expression of NKG2D, an NK cell receptor, was increased. The increase of lytic rate of Yac-1 in NK cells was positively correlated with NK cell activity and NKG2D activity. Conclusion: Blocking TGF-β1 receptor in vitro can increase T lymphocyte immunity and thus resist the infection of Echinococcus granulosus.