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采用日本血吸虫来成熟卵抗原（SIEA）免疫BALB／c小鼠，制备抗SIEA单克隆抗体。获得一株强阳性、高特异的抗未成熟虫卵的单抗（2B2），免疫球蛋白为IgG1类。免疫印迹分析表明该单抗单一识到日本血吸虫未成熟虫卵抗原中的50kDa分子。免疫荧光染色表明，与雌虫子宫内虫卵呈现强阳性反应，而不与成虫抗原交叉。
BALB / c mice were immunized with Schistosoma japonicum to mature egg antigen (SIEA) to prepare anti-SIEA monoclonal antibody. Obtain a strong positive, highly specific anti-immature egg monoclonal antibody (2B2), immunoglobulin IgG1 class. Western blot analysis showed that the McAb was uniquely identified as a 50 kDa molecule in immature egg antigen of Schistosoma japonicum. Immunofluorescence staining showed that there was a strong positive reaction with female uterine eggs, but not with adult antigens.