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从肯尼亚的学生中分离出M_(24)、M_(25)、K_(36)、K_(34)、K_(39)和K_(91)等6株恶性疟原虫,经Trager等的改良法在体外长期的连续培养观察,测出前3株对乙胺嘧啶敏感,后3株对乙胺嘧啶有抗性。在正常的(内含7.29×10~(-6)M PABA和2.27×10~(-6)M叶酸)和改良的(不含PABA,叶酸量相当于平均生理血浆的叶酸浓度2.27×10~(-8)M)RPMI-1640培养液中继续培养这6株原虫。当原虫生长率每48小时达3倍以上时,用放射性同位素技术测定乙胺嘧啶、环氯胍、M&B_(35769)的抗疟活性。药液用不含PABA和叶酸的培养液
Six P. falciparum isolates such as M_ (24), M_ (25), K_ (36), K_ (34), K_ (39) and K_ (91) were isolated from Kenyan students. Long-term continuous culture in vitro observed that the first three strains were sensitive to pyrimethamine, the latter three strains were resistant to pyrimethamine. In the normal (containing 7.29 × 10 ~ (-6) M PABA and 2.27 × 10 ~ (-6) M folic acid) and modified (without PABA, folic acid equivalent to the average physiological plasma concentration of folic acid 2.27 × 10 ~ (-8) M) RPMI-1640 culture medium to continue the six protozoa. Anti-malarial activity of pyrimethamine, cycloguanidine, M & B_ (35769) was determined using radioisotope techniques when protozoa growth rate reached more than 3 times per 48 hours. Liquid with no PABA and folate culture medium