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用~(60)Co—γ射线3—4万伦琴,辐射处理8个西瓜品种干种子,有7个品种获得易位体。M_1花粉半败育株中有25—33.3％的个体为易位体。其方法是:先对M_1代进行育性鉴定,确定半败育株,再对半败育雄花进行染色体鉴定,选出染色体易位杂合体,同株套袋自交。在易位杂合体M_2株系中,选取正常育性株或其后代M_3作父本或母本,同普通二倍体西瓜品种测交。从测交F_1代的379个组合中,鉴定出9个极少籽组合,52个少籽组合。
With ~ (60) Co-γ rays 3-4000 Roentgen, 8 watermelon cultivars were treated with radiation to dry seeds, and 7 cultivars were translocated. 25-33.3% of the individuals in M_1 pollen semi-abortive plants are transposable. The method comprises the following steps of: first, identifying the M_1 generation for fertility identification, determining the semi-aborted plant, and then identifying the chromosome of the aborted male flower, selecting the chromosome translocation heterozygote and bagging the same strain for selfing. In the translocation heterozygous M_2 line, normal fertile plants or their offspring M_3 were selected as male or female parents and crossed with common diploid watermelon varieties. From the 379 combinations of F_1 generation, 9 fewer seed combinations and 52 fewer seed combinations were identified.