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目的检测辅助性T细胞(Th17细胞)与调节性T细胞(Treg细胞)相关细胞因子白介素(IL)-2、IL-6、IL-10、IL-17、IL-23、转化生长因子-β(TGF-β)在强直性脊柱炎(AS)患者外周血血清中的表达,探讨这6种细胞因子在AS发病中的意义及其与AS临床特征的相关性。方法采用酶联免疫吸附法(ELISA)检测50例AS患者和43例健康对照外周血血清中Th17与Treg相关细胞因子的含量。结果 AS患者血清中的IL-6、IL-17、IL-23含量明显高于健康对照组。AS患者血清各细胞因子与疾病活动度(BASDAI)、红细胞沉降率(ESR)、C反应蛋白(CRP)无相关性。结论 AS患者外周血血清中IL-6、IL-17、IL-23呈高表达,3种炎性细胞因子血浆水平的异常升高可能参与了AS的发病和病变进展。
Objective To detect the expressions of interleukin (IL) -2, IL-6, IL-10, IL-17, IL-23 and TGF-β in T helper T cells (Th17 cells) and regulatory T cells (TGF-β) in the peripheral blood serum of patients with ankylosing spondylitis (AS), and to explore the significance of these six cytokines in the pathogenesis of AS and its correlation with the clinical features of AS. Methods Serum levels of Th17 and Treg in 50 AS patients and 43 healthy controls were detected by enzyme linked immunosorbent assay (ELISA). Results Serum levels of IL-6, IL-17 and IL-23 in AS patients were significantly higher than those in healthy controls. There was no correlation between serum cytokines and disease activity (BASDAI), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) in AS patients. Conclusion Serum levels of IL-6, IL-17 and IL-23 in AS patients are highly expressed. The abnormally elevated plasma levels of the three inflammatory cytokines may be involved in the pathogenesis and progression of AS.