【摘 要】
：
目的探讨抗癌药物华蟾素与丝裂霉素的联合作用诱导Hela细胞发生凋亡的机制。方法体外培养人宫颈癌Hela细胞,以不同浓度的华蟾素与丝裂霉素刺激Hela细胞,倒置显微镜下观察细胞
【机 构】
：
湖南省郴州市第四人民医院,湖南省华容县人民医院,湘南学院医学形态学教研室,
论文部分内容阅读
目的探讨抗癌药物华蟾素与丝裂霉素的联合作用诱导Hela细胞发生凋亡的机制。方法体外培养人宫颈癌Hela细胞,以不同浓度的华蟾素与丝裂霉素刺激Hela细胞,倒置显微镜下观察细胞形态,MTT法检测细胞的增殖,凝胶电泳检测细胞DNA片段化,FITC-Annexin V检测细胞凋亡的发生,RT-PCR检测TNF-α的表达。结果经药物作用后的细胞在倒置显微镜下观察,6 h后开始出现明显的凋亡现象;电泳结果呈200 bp大小的典型梯状带;FITC-Annexin V检测到凋亡早期细胞膜呈强绿色荧光,12 h后胞核逐渐被碘化丙啶染成红色,24 h后大部分细胞被染成红色;RT-PCR产物大小为299 bp。结论华蟾素和丝裂霉素能使细胞生长受到明显的抑制,能诱导Hela细胞发生凋亡,能诱导DNA片段化和TNF-α的表达。
其他文献
During the 1st and 2nd stages of the commissioning of the upgrade project of the Beijing Electron Positron Collider(BEPC Ⅱ),which started on Nov.12,2006 and Oc
We derive some strong type and weak type weighted norm estimates which relate the commutators of potential integral operators to the corresponding maximal opera
采用热重–差式扫描量热法(TG-DSC)分析了不同酸度的盐酸–水合氯化铈饱和溶液中生成的CeCl3·nH2O晶体在空气氛围热分解脱水的过程,热分析表明酸度对CeCl3·nH2O晶体结晶形
Using an isospin-dependent quantum molecular dynamics (IQMD) model, we study the 15C induced reactions from 30-120 MeV/nucleon systematically. Here the valence
Theoretical α-decay half-lives of the heaviest nuclei are calculated using the experimental Qα value. The barriers in the quasi-molecular shape path is determ
Based on the optimized design of the lattice for therapy synchrotron and considering the requirement of radiation therapy,the third order resonant extraction is
氧化/抗氧化失衡是慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)主要发病机制之一.过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated rec
Suppose that function f(z) is transcendental and meromorphic in the plane. The aim of this work is to investigate the conditions in which differential monomials
The high-spin states of 159Lu were populated by fusion-evaporation reaction 144Sm (19F, 4n) with beam energy 106 MeV. A new level scheme was established, which
Electron Cloud Instability has been studied in the operation of BEPC.The BEPC Ⅱ began the commissioning in November 2006 and the positron beam current has reac