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肝细胞再生刺激因子(hepatic stimulator substance,HSS)是一种蛋白质,能从正常断乳鼠、成龄大鼠以及其他哺乳动物的再生性肝脏的匀浆中提取,利用超滤,柱层析和快速蛋白液相色谱层析法可获得其不同的活性蛋白组份。HSS抗酸,耐碱,具有热稳定性(100°15分钟)。其作用有器官特异性,无种族特异性。HSS的产生受到昼夜节律变化的调控。在体外,HSS能促进培养中的肝细胞及肝细胞源性肿瘤细胞的增殖,在体内,能使部分肝切后大鼠,猪及犬肝细胞中的DNA合成速率增加。用于治疗D-氨基半乳糖中毒鼠的肝衰,不仅有助于受损肝细胞的修复,而且能大大提高其生存率,故HSS可望用于临床治疗急性肝功能衰竭。
Hepatic stimulator substance (HSS) is a protein that can be extracted from the homogenates of normal liver, adult rat and other mammalian regenerated liver. Ultrafiltration, column chromatography and The different active protein fractions can be obtained by flash chromatography. HSS acid, alkali, with thermal stability (100 ° 15 minutes). Its role organ-specific, non-race-specific. The production of HSS is regulated by circadian changes. In vitro, HSS can promote the proliferation of hepatocytes and hepatocyte-derived tumor cells in culture. In vivo, HSS can increase the DNA synthesis rate of some hepatectomized rats, pigs and canine hepatocytes. Used to treat D-galactosamine poisoning in rats with liver failure, not only contribute to the repair of damaged liver cells, but also greatly improve its survival rate, so the HSS is expected to be used for clinical treatment of acute liver failure.