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目的:研究益气活血为组方原则的“三芪口服液”对实验性肾炎大鼠肾小管间质的影响,并初步探讨其作用机制。方法:采用葡萄球菌肠毒素B(SEB)分次静脉注射,牛血清白蛋白(BSA)隔日灌胃、弗氏佐剂及BSA分次皮下注射,延长造模时间等方法改进大鼠系膜增生性肾小球肾炎模型。予益气活血中药复方三芪口服液治疗,观察对大鼠的病理形态学、24h尿蛋白定量、尿微量白蛋白、NAG、CysC的影响;并通过应用免疫组化、PCR等技术检测HGF、TGF-β1等细胞因子的。结果:益气活血中药复方三芪口服液能减少大鼠尿微量白蛋白、NAG酶、CysC水平;提高大鼠肾组织HGF表达,减少TGF-β1的表达。结论:益气活血中药复方三芪口服液具有肾小管功能保护作用,调节HGF、TGF-β1的表达可能是其作用机制之一。
Objective: To study the effect of “Qiqi Huoxue” as principle of “Qiqi Oral Liquid” on tubulointerstitial in rats with experimental nephritis, and to explore its mechanism. Methods: Mesenteric hyperplasia was induced by subcutaneous injection of staphylococcal enterotoxin B (SEB), intragastric administration of bovine serum albumin (BSA) every other day, subcutaneous injection of Freund’s adjuvant and BSA, prolongation of modeling time Glomerulonephritis model. To observe the effect on the pathomorphology, 24h urinary proteinuria, urine microalbumin, NAG, CysC in rats treated with Sanqi oral liquid; And HGF was detected by immunohistochemical and PCR techniques, TGF-β1 and other cytokines. Results: Yiqi Huoxue Sanqi oral liquid can reduce urinary microalbumin, NAG enzyme and CysC levels in rats; increase HGF expression and decrease TGF-β1 expression in rat kidney tissue. Conclusion: Yiqi Huoxue Sanqi oral liquid has the function of protecting the renal tubules and regulating the expression of HGF and TGF-β1 may be one of its mechanisms.