论文部分内容阅读
本文探讨小鼠或大鼠一次性sc吗啡对分裂原诱导的淋巴细胞增殖反应的影响。结果,吗啡抑制Con A诱导的小鼠或大鼠全血淋巴细胞增殖反应,作用呈浓度依赖关系;但脾淋巴细胞反应性并未明显受损,即使吗啡给药浓度增至50 mg·kg~(-1)。如分离大鼠全血单个核细胞,再观察其对Con A的反应性,则吗啡的抑制活性依然存在,提示全血中的血浆成分不是全血和脾淋巴细胞对吗啡作用敏感性不同的原因。进一步实验发现,纳洛酮10 mg·kg~(-1)预处理可完全拮抗吗啡25 mg·kg~(-1)所致小鼠和大鼠全血淋巴细胞反应性下降,使其恢复至正常水平;而单独给予纳洛酮,对脾和全血淋巴细胞增殖皆无影响。结果表明,吗啡整体给药的免疫调节作用与药物剂量及淋巴细胞的组织来源有关,且其效应通过阿片受体介导。
This article explores the impact of one-time sc-morphine in mice or rats on mitogen-induced lymphocyte proliferation. As a result, morphine inhibited the proliferation of mouse or rat whole blood lymphocytes induced by Con A in a concentration-dependent manner, but the spleen lymphocyte reactivity was not significantly impaired. Even though morphine administration increased to 50 mg · kg ~ (-1). Such as isolation of rat whole blood mononuclear cells, and then observe the reactivity of Con A, morphine inhibitory activity still exists, suggesting that the plasma composition of whole blood is not the sensitivity of whole blood and spleen lymphocytes to morphine causes different . Further experiments showed that naloxone 10 mg · kg -1 could completely antagonize the decline of reactivity of whole blood lymphocytes of mice and rats induced by morphine 25 mg · kg -1 Normal levels; Naloxone, alone, had no effect on spleen and whole blood lymphocyte proliferation. The results show that the overall immunomodulatory effect of morphine administration is related to the dose of the drug and the tissue source of lymphocytes, and its effect is mediated by opioid receptors.